Effects of varying environment on production of biologically active compounds in neem

Bhavya Arora, Narender Chaudhry , Rashmi Mittal


This article is focused on preparation of different extracts from the leaves of Azadirachta indica through soxhlet extraction method using six different types of solvents and determine their total flavonoids content, antimicrobial and larvicidal activity from different places of Haryana. UV–visible spectroscopy method was used to identify the total flavonoids content and on the other hand agar well diffusion method which was used against Escherichia coli (E. coli) to determine the antimicrobial activity of different crude extracts from the leaves of Azadirachta indica. The range of the total flavonoid content of different leave extract was found to be 61.50–529.50 mg/100 g powder samples. The working concentrations of antimicrobial and larvicidal activity is attributed differently by the soxhelt extraction method for the extraction of all crude extracts from Azadirachta indica. At last it was concluded that all crude extracts prepared by solvents of different polarities support their use as medicine for treating several diseases.

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